Are these lesions blocking DNA replication, and therefore lethal to the cell, or not? And if not, are they mutagenic? In collaboration with the University of Aix-Marseille, these probes were transfected into cells in culture. It was shown that lesions due to small adducts (methyl) were efficiently repaired, while larger adducts (hydroxyethyl and above) created highly mutagenic lesions.
609 The O6-hydroxyethylGuanine lesion.