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Thierry Douki

Publié le 3 octobre 2020
17 avenue des Martyrs
38054 Grenoble Cedex 9; France

1988-1989: Master in organic synthesis (Lyon, France)
1986-1989: Engineering School of Chemistry (Lyon, France)
1983-1985: University School of Technicians in Chemistry (Lyon, France)

Research and professional experience
Since November 1993: senior scientist at the Atomic Energy Commission, CEA-Grenoble; France current position: deputy-head of the SyMMES laboratory, researcher in the CIBEST team.
1992-1993: Post-doctoral fellowship, Prof. Bruce Ames' laboratory (UC Berkeley, California, USA) topic: Genotoxic effects of nitric oxide and lipid peroxidation
1989-1992: Thesis in chemistry, supervisor Jean Cadet (CEA Grenoble) topic: Far-UV photochemistry of DNA models

Scientific Interests
My work in toxicology aims at linking results from test tube experiments with those of cellular studies. Analytical chemistry is very powerful for this purpose.
1. Photobiology. A large fraction of my activity deals with the formation of UV-induced photodamage in DNA. A main breakthrough was the development in the early 2000’s of a HPLC-mass spectrometry-based assay for the individual quantification of all possible photoproducts. This made possible the collection of novel data on the photochemistry of double-stranded DNA as well as major applications in mammalian cells. In particular, my work established that the UVA range of sunlight generates some of the photoproducts believed to be due only to UVB. I also gathered new information on DNA repair and worked with the cosmetics industry on photoprotection. In addition, I got involved in collaborative works in projects on microorganisms.
2. Formation of adducts by covalent binding of organic compounds to DNA. My first interest in this field was adducts of endogenous compounds like those produced by lipid peroxidation. More recently, I studied the formation of DNA adducts, in skin and in internal organs, in mice topically exposed to sulfur mustard. Currently, my main interest in chemical genotoxicity is the formation of DNA adducts to polycyclic aromatic hydrocarbons. I am in particular interested in identifying the factors modulating metabolism and genotoxicity of these compounds like mixture effects and co-exposure with sunlight. Assays are also available for oxidatively generated DNA lesions that could also be applied to works on oxidative stress like that induced by nanoparticles.
3. Development of analytical tools for the quantification of biomarkers. My most recent research topic is the development of HPLC-MS/MS methods for the quantification of specific molecular biomarkers of exposure in biological fluids. For example, we are working on the quantification of DNA and glutathione derivatives produced upon exposure to chemical warfare agents like sulfur mustard. The biomarkers are quantified in culture media, plasma and urine by a HPLC-MS/MS technique including isotopic dilution and on-line solid phase extraction.

Scientific production
200 original research articles, 40 review articles and 30 book chapters (H-index: 65).

2018: Research Award of the American Society for Photobiology
2016: Photon Award for the most cited review in the journal of the American Society for Photobiology
2006: New investigator Award of the American Society for Photobiology
1995: Young Scientist Award of the French Society for Photobiology

Scientific activities
Expert for ANSES (French Agency for Food, Environmental and Occupational Health & Safety)
Member of the American, European and French (President) Societies for Photobiology
Member of the French Society for Genetic Toxicology
Associate editor for Photochemistry and Photobiology

Selection of recent publications
(1) Douki, T. (2020) Oxidative stress and genotoxicity in melanoma induction: impact on repair rather than formation of DNA damage? Photochem Photobiol.
(2) Douki, T. (2020) Pyrimidine (6‐4) pyrimidone photoproducts in UVA‐irradiated DNA: photosensitization or photoisomerization? ChemPhotoChem 4, 294-299.
(3) Young, A. R., Greenaway, J., Harrison, G. I., Lawrence, K. P., Sarkany, R., Douki, T., Boyer, F., Josse, G., Questel, E., Monteil, C. and Rossi, A. B. (2018) Sub-optimal Application of a High SPF Sunscreen Prevents Epider-mal DNA Damage in Vivo. Acta Derm. Venereol. 98, 880-887.
(4) Josse, G., Douki, T., Le Digabel, J., Gravier, E. and Questel, E. (2018) The use of suction blisters to measure sunscreen protection against UVR-induced DNA damage. J. Photochem. Photobiol. B: Biol. 179, 1-6.
(5) Premi, S., Wallisch, S., Mano, C. M., Weiner, A. B., Bacchiocchi, A., Wakamatsu, K., Bechara, E. J. H., Halaban, R., Douki, T. and Brash, D. E. (2015) Chemiexcitation of melanin derivatives induces DNA photoproducts long after UV exposure. Science 347, 842-847.
DNA adducts
(1) von Koschembahr, A., Youssef, A., Beal, D., Bourgart, E., Rivier, A., Marques, M., Leccia, M. T., Giot, J. P., Maitre, A. and Douki, T. (2020) Metabolism and genotoxicity of polycyclic aromatic hydrocarbons in human skin explants: mixture effects and modulation by sunlight. Arch Toxicol 94, 495–507.
(2) von Koschembahr, A., Youssef, A., Béal, D., Calissi, C., Bourgart, E., Marques, M., Leccia, M.-T., Giot, J.-P., Maitre, A. and Douki, T. (2018) Solar simulated light exposure alters metabolization and genotoxicity induced by benzo[a]pyrene in human skin. Sci. Rep. 8, 14692.
(3) Genies, C., Maître, A., Tarantini, A. and Douki, T. (2014) Evaluation in vitro de la génotoxicité des hydrocarbures aromatiques polycycliques purs ou en mélange. Environ. Risques Santé 13, 318-324.
(4) Batal, M., Boudry, I., Mouret, S., Clery-Barraud, C., Wartelle, J., Berard, I. and Douki, T. (2014) DNA damage in internal organs after cutaneous exposure to sulphur mustard. Toxicol. Appl. Pharmacol. 278, 39-44.
(5) Batal, M., Boudry, I., Mouret, S., Wartelle, J., Emorine, S., Bertoni, M., Berard, I., Clery-Barraud, C. and Douki, T. (2013) Temporal and spatial features of the formation of DNA adducts in sulfur mustard-exposed skin. Toxicol. Appl. Pharmacol. 273, 644-650.

Link to the complete list of publications.