Thesis presented December 08, 2020

Abstract:
Today, early diagnosis of cardiac pathologies is a major issue in healthcare world. Indeed, the speed of myocardial infarction diagnosis has an impact not only on the patient's health, but also on the management of emergency hospital services. The use of diagnostic devices at the patient’s bedside is a relevant solution to overcome effectively such a challenge. Consequently, the number of Point-Of-Care systems dedicated to the diagnosis of cardiac pathologies is growing. However, these devices have some disadvantages that need to be overcome.
This thesis work has been conducted in this context. Research and development of an innovative method for the detection of cardiac biomarkers has been carried out. The objective of this method is the detection of any type of analyte in a complex medium with a good sensitivity allowed by the biomolecular amplification used. This generic method is based on the LAMP amplification of an oligonucleotide probe. It uses aptamer probes, specific to the target to be detected, which have been validated by surface plasmon resonance imaging. This method has been implemented in a relevant manner on different models and applied to the detection of a cardiac biomarker of interest, troponin I. The integration of this method in a portable microfluidic device was finally addressed for future use in field diagnostics.

Keywords:
troponin, aptamer, LAMP amplification, cardiac pathologies, aptamero-LAMP