Thesis presented July 12, 2004
Abstract:
The NADPH oxidase complex of neutrophils catalyses the formation of superoxyde ions involved in the elimination of pathogenes. It contains two membrane proteins (gp91phox, p22phox), three cytosolic factors (p47phox, p67phox, p40phox), and a small G protein, Rac. In order to understand interactions and structural modifications, structural studies of full-length cytosolic factors alone and complexed have been undertaken. Two approaches have been selected : electron microscopy and small angle X-ray scattering. The shape of p47phox and p67phox have been calculated using X-ray scattering.
Keywords:
NADPH oxidase complex, cytosolic factors, electron microscopy, small-angle X-ray scattering
On-line thesis.